Analysis Note

ControlTested on Rat Salivary Gland Lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Aquaporin 5 in rat lung and mouse trachea tissue.
Immunohistochemistry Analysis: A representative lot from independent laboratories detected Aquaporin 5 in various paraformaldehyde fixed tissues (see references).

This Anti-Aquaporin 5 (AQP5) Antibody is validated for use in WB for the detection of Aquaporin 5 (AQP5).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aquaporins (AQPs) are a family of channel proteins that allow water or very small solutes to pass, functioning in tissues where the rapid and regulated transport of fluid is necessary, such as the kidney, lung, and salivary glands. Aquaporin 5 is considered to play an important role in water movement across the plasma membrane in salivary fluid secretion and corneal/lens lubrication and is a focus of studies illucidating bechanisms underlying diabetic xerostomia.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat Aquaporin 5.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

ImmunoAffinity Purified

Quality

Evaluated by Western Blotting in rat salivary gland tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Aquaporin 5 in 10 µg of rat salivary gland tissue lysate.

Specificity

Aquaporin 5 (AQP5). By Western blot the antibody reacts with a band at ~ 24 kDa on rat submaxillary gland lysate.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Target description

~28 kDa observed